A Secret Weapon For Ligaz
A Secret Weapon For Ligaz
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All archaeal proteomes contain an ATP-dependent DNA ligase that resembles in all structural respects (amino acid sequence; conservation of NTase motifs, such as motif VI; and tertiary construction composed of the N-terminal DBD plus the central NTase and C-terminal OB domains) (twenty, 21) the canonical ATP-dependent DNA ligases of eukaryal cells. Biochemical characterization of various archaeal DNA ligases confirmed their demanding dependence on ATP and failure to use NAD+ (summarized in Ref. 22). Against this, other archaeal ligases have been described to have “twin specificity,” variously employing ATP and
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(eighteen) have carried out a Digital screen for small molecules which could dock to the N-terminal DBD of HuLig1, a website conserved in all mammalian DNA ligases. They thereby discovered compounds that (i) inhibited nick sealing by human ligases in vitro
, involving the NTase and HhH domains. Determined by out there structural data, it is evident that DNA ligases have developed not less than three unique indicates of encircling DNA.
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